Developed methods for routine analysis of medicines should be considered in terms of\nanalytical effciency, economic cost, as well as their environmental impact. Different chromatographic\nmethods for the routine quantitative analysis of hederacoside C in ivy leaf extract and its original\ndosage forms (capsules and syrup) are developed. The performance of HPLC and UPLC methods\nshould be done using ACE C18 (150 mm x 4.6 mm, 5.0 microM) and ACQUITY UPLC BEH C18\n(50 mm x 2.1 mm, 1.7 microM) columns, respectively, and both of them require a mixture of water\nand acetonitrile in the ratio 71/29 as a mobile phase. The HPTLC procedure is carried out using\nthe stationary phase pre-coated silica gel 60 F254 glass sheets and a mixture of anhydrous formic\nacid/acetone/methanol/ethyl acetate (4:20:20:30 v/v). The most suitable conditions of preparation\nfor each sample are established, for instance, the solid-phase extraction (SPE) for the analysis of\nsyrup is applied. Analytical methods are compered by analytical accuracy, calculation of expenses,\nand assessment of their influence on ecology. All methods are recognized as accurate, precise,\nand reliable. However, the assessment of the environmental impact shows that HPTLC is the less\ngreen method. On the another hand, it is found to be the cheapest, the costs of performing HPTLC\nare 2.3 and 1.6 times lower than for HPLC and UPLC, respectively.
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